Phenotype produced by transient overexpression or silencing of FvRLP2 in strawberry fruits and Arabidopsis plants. (A) Lesion development after inoculation of strawberry fruits with Botryti cinerea. DAY-1 indicated the first day after fruit were injected with Agrobacterium containing overexpression or silencing constructs of FvRLP2. DAY-3 indicated the first day after inoculation with B. cinerea. AS, silencing vector; EV, overexpression empty vector; OE, overexpression vector. (B) Measurement of lesion area using ImageJ. (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01). (C) Relative expression levels of FaPR1 in fruit from different treatments (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01). (D) Leaf phenotypes of transgenic Arabidopsis overexpressing FvRLP2 and wild-type control plants during infection by B. cinerea. (E) Trypan blue staining revealed cell death at 48 h after infection. (F) Measurement of lesion area using ImageJ (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01). (G) Relative expression levels of AtPR1 in the transgenic lines, respectively (n = 10, Student’s t-test, *P < 0.05 and **P < 0.01).