Credit: (Image courtesy of Sarah Yannarell | Shank Lab | University of North Carolina, Chapel Hill)
A team of scientists led an experimental study to determine the relationships between subpopulations of cells within the biofilm of a model microbe, revealing new insights regarding their potential. These images show top-down views of colonies of Bacillus subtilis grown on agar plates. The image on the left is a brightfield image while the one on the right shows false-color fluorescence of this colony. These bacterial cells contain two reporter constructs that cause the cells to produce two distinct fluorescence signals (shown as green and pink) when one or both of those genes are expressed.